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flag 66008 4 ig  (Proteintech)


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    Structured Review

    Proteintech flag 66008 4 ig
    Flag 66008 4 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag+66008+4+ig/UBR5+Antibody/pmc12765265-62-17-17
    Average 94 stars, based on 21 article reviews
    flag 66008 4 ig - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: CLOCK promotes proliferation of glioblastoma cells through acetylating PRPS1/2.
    Article Snippet: Purpose The oncogenic impairment of the circadian clock plays an important role in tumorigenesis.. However, it remains unclear whether the activation of epidermal growth factor (EGF) receptor (EGFR), which plays a critical role in glioblastoma (GBM) development, regulates the circadian clock and thereby tumorigenesis.. Methods This study employed molecular techniques including DNA mutagenesis, qPCR, immunoprecipitation, immunofluorescence, and functional assays.

    Western Blot:

    Article Title: Targeting EGFR-binding protein SLC7A11 enhancing antitumor immunity of T cells via inducing MHC-I antigen presentation in nasopharyngeal carcinoma.
    Article Snippet: .. Western blotting was conducted in accordance with a previously established protocol [25], using the following antibodies: SLC7A11 (1:1000, ab175186, Abcam, 35-55kD; 1:1000, 26864-1-AP, Proteintech Group, 55kD; 1:1000, 382036, Zenbio, 55kD), EGFR (1:2000, 26864-1-AP, Proteintech Group), p-EGFR (1:1000, R24173, ZenBioScience), Flag (1:2000, 66008-4-Ig,Proteintech Group), GR (1:5000, 66904-1-Ig, Proteintech Group), TAP1 (1:3000, 11114-1-AP, Proteintech Group), FAF2 (1:3000, 16251-1-AP, Proteintech Group), MHC-I (1:2000, 15240-1-AP, Proteintech Group), SYVN1 (1:1000, 13473-1-AP, Proteintech Group), OS-9 (1:1000, 10061-1-AP, Proteintech Group), VCP (1:2000, 10736-1-AP, Proteintech Group), HERP (1:1000, 10813-1-AP, Proteintech Group), Ubiquitin (1:1000, #3936, CST), β-actin (1:3000, AF7018, Affinity), Tubulin (1:10000, AC021, Abclonal), Histone H3 (1:2000, 68345-1-Ig, Proteintech Group). ..

    Immunopeptidomics:

    Article Title: Targeting EGFR-binding protein SLC7A11 enhancing antitumor immunity of T cells via inducing MHC-I antigen presentation in nasopharyngeal carcinoma.
    Article Snippet: .. Western blotting was conducted in accordance with a previously established protocol [25], using the following antibodies: SLC7A11 (1:1000, ab175186, Abcam, 35-55kD; 1:1000, 26864-1-AP, Proteintech Group, 55kD; 1:1000, 382036, Zenbio, 55kD), EGFR (1:2000, 26864-1-AP, Proteintech Group), p-EGFR (1:1000, R24173, ZenBioScience), Flag (1:2000, 66008-4-Ig,Proteintech Group), GR (1:5000, 66904-1-Ig, Proteintech Group), TAP1 (1:3000, 11114-1-AP, Proteintech Group), FAF2 (1:3000, 16251-1-AP, Proteintech Group), MHC-I (1:2000, 15240-1-AP, Proteintech Group), SYVN1 (1:1000, 13473-1-AP, Proteintech Group), OS-9 (1:1000, 10061-1-AP, Proteintech Group), VCP (1:2000, 10736-1-AP, Proteintech Group), HERP (1:1000, 10813-1-AP, Proteintech Group), Ubiquitin (1:1000, #3936, CST), β-actin (1:3000, AF7018, Affinity), Tubulin (1:10000, AC021, Abclonal), Histone H3 (1:2000, 68345-1-Ig, Proteintech Group). ..

    Ubiquitin Proteomics:

    Article Title: Targeting EGFR-binding protein SLC7A11 enhancing antitumor immunity of T cells via inducing MHC-I antigen presentation in nasopharyngeal carcinoma.
    Article Snippet: .. Western blotting was conducted in accordance with a previously established protocol [25], using the following antibodies: SLC7A11 (1:1000, ab175186, Abcam, 35-55kD; 1:1000, 26864-1-AP, Proteintech Group, 55kD; 1:1000, 382036, Zenbio, 55kD), EGFR (1:2000, 26864-1-AP, Proteintech Group), p-EGFR (1:1000, R24173, ZenBioScience), Flag (1:2000, 66008-4-Ig,Proteintech Group), GR (1:5000, 66904-1-Ig, Proteintech Group), TAP1 (1:3000, 11114-1-AP, Proteintech Group), FAF2 (1:3000, 16251-1-AP, Proteintech Group), MHC-I (1:2000, 15240-1-AP, Proteintech Group), SYVN1 (1:1000, 13473-1-AP, Proteintech Group), OS-9 (1:1000, 10061-1-AP, Proteintech Group), VCP (1:2000, 10736-1-AP, Proteintech Group), HERP (1:1000, 10813-1-AP, Proteintech Group), Ubiquitin (1:1000, #3936, CST), β-actin (1:3000, AF7018, Affinity), Tubulin (1:10000, AC021, Abclonal), Histone H3 (1:2000, 68345-1-Ig, Proteintech Group). ..

    Article Title: TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis.
    Article Snippet: Primary antibodies against TC2N (Cat. #: HPA027549) was purchased from Sigma-Aldrich. .. Flag (Cat. #: 80010-1-RR and 66008-4-Ig), FASN (Cat. #: 10624-2-AP and 66591-1-Ig), TRIM21 (Cat. #: 12108- 1-AP and 67136-1-Ig), PTEN (Cat. #: 22034-1-AP and 60300-1-Ig), LaminA (Cat. #: 10298-1-AP), α-Tubulin (Cat. #: 11224-1-AP), importinβ (Cat. #: 10077-1-AP and 675971-Ig), NEDD8 (Cat. #: 16777-1-AP), NANOG (Cat. #: 14295-1-A), SOX2 (Cat. #: 11064-1-AP) and Ubiquitin (Cat. #: 10201-2-AP) were purchased from Proteintech Group (Wuhan, China). .. IPO5 (Cat. #: ab187175) was purchased from Abcam (Cambridge, MA, USA).



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    1,8-Cineole reduces the ubiquitinated degradation of peroxisome proliferator-activated receptor-γ (PPAR-γ) at Lys-466. (A) After treating human aortic endothelial cells (HAECs) with high palmitic acid and high glucose (PA/HG) and/or 1,8-cineole in the presence of cycloheximide (CHX; 10 μg/mL) for 0, 3, 6, 9, 12, or 24 h, the protein level of PPAR-γ was assessed using Western blotting analysis ( n = 3). (B) The protein level of PPAR-γ in HAECs was detected with Western blotting analysis after treatment with chloroquine (CQ) or MG132 in the presence of CHX in PA/HG-treated HAECs ( n = 3). (C) 1,8-Cineole decreased the ubiquitination degradation of PPAR-γ induced by PA/HG in HAECs ( n = 3). (D) Ubiquitination sites of PPAR-γ determined by mass spectrometry analysis. (E) Representative Western blotting of HEK 293T cells transfected with HA-Ub and Flag-PPAR-γ-WT or PPAR-γ-K170R, PPAR-γ-K466R, or PPAR-γ-K485R mutants after immunoprecipitation with <t>anti-Flag</t> antibody, verified using HA-tagged, Flag-tagged, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies. Data are represented as mean ± standard deviation (SD). ∗∗ P < 0.01 vs. the control, # P < 0.05 vs. PA/HG, $ P < 0.05 vs. PA/HG + CHX, NS: no significance. 1,8-cineole-H: high-dose 1,8-cineole; Ub: ubiquitin; IgG: immunoglobulin G.
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    Image Search Results


    1,8-Cineole reduces the ubiquitinated degradation of peroxisome proliferator-activated receptor-γ (PPAR-γ) at Lys-466. (A) After treating human aortic endothelial cells (HAECs) with high palmitic acid and high glucose (PA/HG) and/or 1,8-cineole in the presence of cycloheximide (CHX; 10 μg/mL) for 0, 3, 6, 9, 12, or 24 h, the protein level of PPAR-γ was assessed using Western blotting analysis ( n = 3). (B) The protein level of PPAR-γ in HAECs was detected with Western blotting analysis after treatment with chloroquine (CQ) or MG132 in the presence of CHX in PA/HG-treated HAECs ( n = 3). (C) 1,8-Cineole decreased the ubiquitination degradation of PPAR-γ induced by PA/HG in HAECs ( n = 3). (D) Ubiquitination sites of PPAR-γ determined by mass spectrometry analysis. (E) Representative Western blotting of HEK 293T cells transfected with HA-Ub and Flag-PPAR-γ-WT or PPAR-γ-K170R, PPAR-γ-K466R, or PPAR-γ-K485R mutants after immunoprecipitation with anti-Flag antibody, verified using HA-tagged, Flag-tagged, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies. Data are represented as mean ± standard deviation (SD). ∗∗ P < 0.01 vs. the control, # P < 0.05 vs. PA/HG, $ P < 0.05 vs. PA/HG + CHX, NS: no significance. 1,8-cineole-H: high-dose 1,8-cineole; Ub: ubiquitin; IgG: immunoglobulin G.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: 1,8-Cineole ameliorates vascular endothelial senescence in diabetes mellitus by directly targeting and deubiquitinating PPAR-γ in vivo and in vitro

    doi: 10.1016/j.jpha.2025.101307

    Figure Lengend Snippet: 1,8-Cineole reduces the ubiquitinated degradation of peroxisome proliferator-activated receptor-γ (PPAR-γ) at Lys-466. (A) After treating human aortic endothelial cells (HAECs) with high palmitic acid and high glucose (PA/HG) and/or 1,8-cineole in the presence of cycloheximide (CHX; 10 μg/mL) for 0, 3, 6, 9, 12, or 24 h, the protein level of PPAR-γ was assessed using Western blotting analysis ( n = 3). (B) The protein level of PPAR-γ in HAECs was detected with Western blotting analysis after treatment with chloroquine (CQ) or MG132 in the presence of CHX in PA/HG-treated HAECs ( n = 3). (C) 1,8-Cineole decreased the ubiquitination degradation of PPAR-γ induced by PA/HG in HAECs ( n = 3). (D) Ubiquitination sites of PPAR-γ determined by mass spectrometry analysis. (E) Representative Western blotting of HEK 293T cells transfected with HA-Ub and Flag-PPAR-γ-WT or PPAR-γ-K170R, PPAR-γ-K466R, or PPAR-γ-K485R mutants after immunoprecipitation with anti-Flag antibody, verified using HA-tagged, Flag-tagged, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies. Data are represented as mean ± standard deviation (SD). ∗∗ P < 0.01 vs. the control, # P < 0.05 vs. PA/HG, $ P < 0.05 vs. PA/HG + CHX, NS: no significance. 1,8-cineole-H: high-dose 1,8-cineole; Ub: ubiquitin; IgG: immunoglobulin G.

    Article Snippet: Anti-PPAR-γ (60127-1-Ig), anti-P53 (60283-2-Ig), anti-PAI-1 (66261-1-Ig), anti-P21 (82669-2-RR), anti-P16 (10883-1-AP), anti-ubiquitin (10201-2-AP), anti-HA Tag (51064-2-AP), and anti-Flag Tag (66008-4-Ig) antibodies were procured from Proteintech (Wuhan, China); anti-γ-H2A.X (ab81299) antibody from Abcam (Danvers, MA, USA); and anti-GAPDH (MB001) antibody from Bioworld (Nanjing, China).

    Techniques: Western Blot, Ubiquitin Proteomics, Mass Spectrometry, Transfection, Immunoprecipitation, Standard Deviation, Control